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evagreen qpcr dye  (Biotium)


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    Biotium evagreen qpcr dye
    Evagreen Qpcr Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/evagreen+qpcr+dye/pm42015259-239-13-16?v=Biotium
    Average 97 stars, based on 3221 article reviews
    evagreen qpcr dye - by Bioz Stars, 2026-08
    97/100 stars

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    A. The 4-plex assay (4+G) shows a higher accumulated methylation signal (indicated by a lower mean Cq value) compared to the singleplex assay (1+G) for the positive control (hypermethylated DNA). B . qPCR readout of the 4-plex (blue) and 1-plex (purple) assays on the positive control (hypermethylated DNA). ROX (red) is used as a reference dye, and GAPDH (green) is used to report DNA input quantity and normalize methylation detection.

    Journal: bioRxiv

    Article Title: Systematic design of multiplex methylation-specific qPCR for cancer related biomarkers

    doi: 10.1101/2024.12.09.627654

    Figure Lengend Snippet: A. The 4-plex assay (4+G) shows a higher accumulated methylation signal (indicated by a lower mean Cq value) compared to the singleplex assay (1+G) for the positive control (hypermethylated DNA). B . qPCR readout of the 4-plex (blue) and 1-plex (purple) assays on the positive control (hypermethylated DNA). ROX (red) is used as a reference dye, and GAPDH (green) is used to report DNA input quantity and normalize methylation detection.

    Article Snippet: 10 ng of bisulfite-converted DNA input were used in 10 µL qPCR reactions with 2x EvaGreen qPCR MasterMix with ROX dye (Biotium, Cat#31042).

    Techniques: Plex Assay, Methylation, Singleplex Assay, Positive Control